睡眠剥夺对大鼠脑神经元型一氧化氮合酶mRNA表达的影响

Effect of sleep deprivation on expression of neuronal nitric oxide synthase mRNA in brains of rats

目的 观察快动眼睡眠 (rapideyemovementsleep ,REMS)剥夺大鼠不同脑区神经元型一氧化氮合酶(nNOS)mRNA表达的变化。方法 小站台水环境法剥夺大鼠睡眠 2 4h后 ,用半定量逆转录聚合酶链反应 (RT -PCR)检测其下丘脑、海马、大脑皮层nNOSmRNA的表达。结果 睡眠剥夺 2 4h后大鼠下丘脑和海马的nNOSmR NA表达量显著增高 ,与对照组比较有显著性差异 (P <0 0 1) ;睡眠剥夺 2 4h组大鼠大脑皮层的nNOSmRNA表达量与大站台对照组比较无显著差异。结论 睡眠剥夺 2 4h可显著上调下丘脑和海马nNOSmRNA的表达量 ,可能是睡眠剥夺对神经系统功能复杂影响的机制之一。

Objective To study the effect of rapid eye movement sleep(REMS) deprivation on the expression of mRNA coding for neuronal nitric oxide synthase(nNOS) of different brain regions of rats.Methods The flower pot technique was adopted to deprive the REMS of Sprague-Dawley rats for 24?h.The expression of mRNA coding for nNOS of hypothalamus,hippocampus and cerebral cortex in rats was assayed by reverse-transcription polymerase chain reaction(RT-PCR).Results The expression of nNOS mRNA of hypothalamus and hippocampus in rats increased significantly in group of REMS deprivation for 24 hours(P<0.01).There was no difference in the expression of nNOS mRNA of cerebral cortex among group of REMS deprivation for 24 hours and large-table control group(P>0.05).There was no difference in the expression of nNOS mRNA of cerebral cortex among group of REMS deprivation for 24 hours and large-table control group(P>0.05).Conclusion The expression of nNOS mRNA of hypothalamus and hippocampus can be upregulated markedly after 24?h sleep deprivation,which must be one of the mechanisms of complicated influence of sleep deprivation on functions of central nervous system.