腺病毒介导的基因治疗在骨创伤及骨病中的应用

APPLICATION OF GENE THERAPY MEDIATED BY ADENOVIRUS VECTORS FOR BONE TRAUMA AND BONE DESEASE

目的 介绍腺病毒介导的基因治疗的特点及其在骨创伤和骨病中的应用。 方法 广泛查阅有关重组腺病毒载体的制备原理、特点及应用研究进展等方面的文献 ,并总结腺病毒介导的基因治疗在促进骨愈合、治疗骨质疏松及类风湿性关节炎等骨病研究中的应用情况。 结果 腺病毒载体具有高转染率、目的基因的高表达率及在宿主细胞内的表达有一定的时限等特点 ,能有效转染软骨细胞、成骨细胞、破骨细胞、骨髓基质细胞、关节滑膜细胞、椎间盘髓核细胞及成纤维细胞等 ,适用于骨折愈合、脊柱融合、骨质疏松及类风湿性关节炎等骨病的基因治疗。 结论 腺病毒介导的基因治疗可望为骨创伤、骨病的治疗提供新的途径。

Objective To review the current concepts of gene therapy approaches mediated by adenovirus vectors for bone trauma and bone disease. Methods The recent literature concerned gene therapy mediated by adenovirus vectors was reviewed, which provides new insights into the treatments of bone trauma and bone disease. Results Adenovirus vectors was efficient, achieved high expression after transduction, and could transfer genes to both replicating and nonreplicating cells, such as osteoblasts, osteoclasts, fibroblasts, chondrocytes, bone marrow stromal cells, etc. Gene therapy mediated by adenovirus vectors achieved affirmative results in enhancing bone union and in curing bone diseases, such as osteoporosis and rheumatoid arthritis. Conclusion Gene therapy mediated by adenovirus offers an exciting avenue for treatment of bone trauma and bone diseases.