人耳软骨移植抗原性及脱细胞处理对其的影响

ALLOGRAFT ANTIGENICITY OF HUMAN EAR CARTILAGE AND EFFECT OF CELL EXTRACTION ON ANTIGENICITY

目的 研究人耳软骨的移植抗原性及脱细胞处理对其的影响。 方法 用低渗的 Triton X- 10 0为主的方法脱去人耳软骨细胞 ,然后通过抗人主要组织相容性复合体 - I(MHC- I)免疫组织化学染色 ,与单个核细胞共同培养来研究人耳软骨的移植抗原性及脱细胞处理对其的影响。 结果 软骨细胞胞浆抗人 MHC- I为阳性 ,抗原的分布以细胞核周边的细胞质为主。细胞外基质抗人 MHC- I为阴性。而经过脱细胞处理后的人耳软骨则呈抗人 MHC- I阴性。单个核细胞与软骨共同培养时则显示出较高 DNA合成能力 ,与对照组比较有统计学意义 (P<0 .0 5 )。单个核细胞与脱细胞处理的软骨共同培养后的 DNA合成能力较低 ,与单纯的单个核细胞培养展示出新的 DNA合成能力 ,但无显著差异 (P>0 .0 5 )。单个核细胞与正常人耳软骨共同培养的过程中 ,可见移向软骨的单个核细胞随时间增加而增加。相反 ,单个核细胞与脱细胞处理的软骨共同培养后 ,却少见单个核细胞移向脱细胞处理的软骨 (P<0 .0 1)。 结论 软骨具有移植抗原性 ;以低渗的 Triton X- 10 0为主的脱细胞方法可以有效的去除移植物的抗原性 ,从而避免同种异体组织移植排斥反应的发生。

Objective To study the allograft antigenicity of human ear cartilage and the effect of the cell extraction on antigenicity. Methods The human ear cartilage was acellularized by cell extraction with Triton X-100. Then the cartilage and the acellular cartilage were analyzed by anti-MHC-I immunohistochemical staining, the reaction of the peripheral blood mononuclear(PBM) cells to the cartilage and the acellular cartilage and the migration of the PBM cells toward the cartilage and the acellular cartilage. Results The result of human ear cartilage was positive for the anti-MHC-I immunohistochemical staining, whereas that of the acellular cartilage was negative for the staining. The reactive proliferation of the PBM cells was more when they were co-cultured with human ear cartilage than that when they were cultured alone in vitro( P< 0.05), but the acellular cartilage did not show the same phenomena ( P> 0 05); when the cartilage and the acellular cartilage were co-cultured with the PBM cells, the PBM cells migrated to the cartilage much more than that to acellular cartilage( P< 0.01). Conclusion Human ear cartilage has allograft antigenicity and its antigenicity can be removed by cell extraction with Triton X-100.