封闭负压引流技术对创周组织水肿及血管通透性的影响

The experimental study of the effects of vacuum assisted closure on edema and vessel permeability of the wound

目的创面封闭负压引流技术促进创面愈合的机制尚未清楚,探讨封闭负压引流对创周组织水肿及血管通透性的影响。方法以兔耳背全层皮肤缺损的急性创面为模型,右耳用创面多孔闭合敷料,并给予负压引流,为治疗组;左耳创面不作封闭负压引流,其余处理同治疗组,为对照组。在治疗后第2,4,6,8天分别利用干湿法检测对照组和治疗组创周组织的含水量,同时用伊文氏蓝渗出法检测创周组织的血管通透性。结果封闭负压引流组创周组织含水量在2,4,6,8d均低于对照组(P<0.05);伊文氏蓝含量在第2天两组差异无显著性意义(P>0.05),但在4,6,8d明显低于对照组(P<0.01)。结论封闭负压引流治疗可以有效的减轻创周水肿、降低血管通透性。

Aim To investigate the effects of vacuum assisted closure(V.A.C)for the edema and the vessel permeabi lity of acute wound.Methods An acute skin wound model of rabbit ear was established,and then the right ear was treated wit h V.A.C(treatment group)and the left was treated with normal dressing(control group)for 2,4,6,8days,re-spectively.Total tissue water cont ent in wound was measured by the method of wet-dry subtraction.Evans Blue(EB)staining was used to measure the vessel permeability of the wound.Results Compared with the control group,the content of tissue water in the treatment group decreased signi fi-cantly after 2,4,6,8day treatment (P<0.05).The EB concentration in tissue also decreased greatly after4,6,8day treatment (P<0.01).Conclusion The vessel permeability and edema of the wound can be effi-ciently counteracted by V.A.C.