摘要
利用鸟苷生产菌株枯草芽孢杆菌 (B subtilis) 75 4#,在 5 0L发酵罐成功优化的基础上 ,分别在 1 2M3的中试规模和 1 0 0M3的生产规模进行了放大 ,产苷分别达到 2 9 4g/L和2 1 4g/L ;进而通过过程缩小 (scaledown)方法 ,从代谢流动态变化的角度研究了放大过程中存在的问题 ,发现DO是限制过程放大的另一重要因素 ,据此将 5 0L规模克服代谢流迁移的优化工艺成功放大到生产规模 ,使产苷水平进一步提高了 1 8% ,达 2 5 2g/L。
Based on the optimized technology on 50L scale, B subtilis 754# was used to manufacture guanosine on pilot scale (12M 3) and plant scale (100M 3) to give the production of 29 4g/L and 21 4g/L successively Through scale down the process of plant scale to pilot scale, based on the theory of dynamic metabolic flux, DO was found to be another key factor that limited the scale up process After conquering the DO limitation, the optimized technology was successfully scaled up to plant scale and the production was further increased by 18% to 25 2 g/L
出处
《微生物学通报》
CAS
CSCD
北大核心
2003年第2期61-64,共4页
Microbiology China
基金
上海市曙光计划资助项目 ( 0 1SG2 8)
