摘要
利用大肠杆菌mRNA中存在的一定程度的poly (A)现象 ,利用oligo (dT)与poly(A)特异结合的特性 ,纯化并逆转录mRNA ,并应用RD PCR方法获得了 1 70多条大肠杆菌poly (A)化mRNA的基因片段 ,利用这些片段打印成基因芯片 ,以供后续大肠杆菌的基因表达研究。
mRNA with poly(A) tracts was enriched from total RNA of E coli with oligo(dT) cellulose After reverse transcription was performed using oligo(dT)18 as primer, double stranded cDNA was synthesized More than 170 gene fragments were obtained by RD PCR technique These gene fragments were dotted onto glass slides substrate to make cDNA microarray The cDNA microarray will be used to explore the gene expression profiling in E coli
出处
《微生物学通报》
CAS
CSCD
北大核心
2003年第2期28-32,共5页
Microbiology China
基金
广州市重点科技攻关项目 (No 99 Z 0 2 2 0 1 )
