玉米大斑病菌原生质体的制备与再生

PROTOPLAST PREPARATION AND REGENERATION OF EXSEROHILUM TURCICUM

以玉米大斑病菌 (Exserohilumturcicum) 0 1 0 9 8为供试菌株 ,研究了菌龄、液体培养基、酶系统、酶解时间、稳渗剂对玉米大斑病菌原生质体制备的影响及稳渗剂对原生质体再生的影响。结果表明制备玉米大斑病菌原生质体适宜的条件为 :Fries液体培养基培养分生孢子 2 4h ,1 %Lywallzyme、 1 %Drislase和 1 %Snailase 3种酶溶液混合使用 ,酶解 5h ,0 7mol/LKCl为稳渗剂 ;原生质体再生以 0

The strain 0109 8 of Exserohilum turcicum , caused north leaf blight of maize, was used for studying the effect of the age of hyphae, liquid medium,enzyme system, time of digestion and stabilizer on the protoplast preparation and the effect of stabilizer on the protoplasts regeneration The results indicated that the optimum condition for preparing protoplasts was: the condia cultured in liquid Fries medium for 24 hours, enzyme mixture of 1%Lywallzyme, 1% Snailase and 1% Drislase used to digest the hyphae for 5 hours, 0 7mol/L KCl used as the osmotic stabilizer ; 0 6mol/L sucrose as the osmotic stabilizer for the regeneration of protoplast