尿脱氧吡啶酚与大鼠实验性骨质疏松相关性研究

Relationship between urine deoxypyridinoline level and osteoporosis

目的研究尿脱氧吡啶酚(DPD)含量变化与大鼠发生实验性骨质疏松的相关性,探讨尿脱氧吡啶酚在骨质疏松防治研究中的意义。方法采用维甲酸诱导的大鼠急性骨质疏松模型,以淫羊藿总黄酮进行对抗治疗。分别测定模型组、淫羊藿总黄酮治疗组和对照组的尿DPD、Ca/Cr、P/Cr,血清骨钙素、甲状旁腺素和骨Ca、骨P及骨密度值,并进行统计学分析。结果DPD值在模型组显著上升,在淫羊藿总黄酮治疗组则趋于正常水平。DPD值所反映骨吸收情况与血清骨钙素、甲状旁腺素及骨指标一致,并比Ca/Cr和P/Cr灵敏。结论尿脱氧吡啶酚是一项灵敏快速的特异性骨质疏松诊断与疗效评价指标。

Objective To investigate the relationship between the content of deoxypyridinoline (DPD) in urine and the experimental osteoporosis in rats.Methods The rat osteoporosis was induced by giving retinoic acid, and treated by feeding total flavonoids of Herba Epimedii (HEF) intragastrically at high,medium and low dosages.The content of DPD,Ca,P and Cr in urine and the level of osteocalcin and parathyroid hormone in serum as well as bone mineral density were determined in the model group, treatment group and control group after the treatment was finished.Results The DPD level increased significantly in the model group,compared with the normal control ( P < 0.01),and decreased to normal level in the treatment group.The bone resorption revealed by DPD was consistent with what the serum and bone indexes demonstrated, and were more sensitive than Ca/Cr and P/Cr ratios. Conclusions DPD is a special and sensitive index in urine for diagnosis of osteoporosis.