反相高效液相色谱法测定兔血清中小檗碱浓度

To Determine the Serum Consentration of Berberine in Rabbits in RP-HPLC

目的 :建立前列栓中小檗碱兔血清药物浓度的反相高效液相色谱定量分析方法。方法 :色谱条件为 :固定相 :PlatinumEPS柱 (C810 0A 3μ ,15 0mm× 4 6mm) ,流动相 :乙腈～ 1 12N磷酸盐缓冲液 ( 70∶30 ,v v) ,流速 :0 8ml·min- 1 ,检测波长 345nm ,柱温40℃ ,外标法为定量测定法。结果 :血清小檗碱浓度在 0 72 μg·L- 1 ～ 5 78 98μg·L- 1 的范围内线性关系良好 ,检测限 0 36 μg·L- 1 ,回收率 ( 96 0 6± 4 74) % ,日内、日间精密度为 1 16 %～ 2 18%、0 99%～ 3 0 4%。结论 :本法准确性、灵敏度、专一性高 ,可为小檗碱的体内血清药物浓度分析提供有意义的方法学参考。

Objective:To create a quantitative analysis method of RP-HPLC to determine the serum concentration of berberine from QLS in rabbits.Methods:The conditions of chromatography:stationary phase:Platinum EPS column (C8 100A 3μ,150mm×4.6mm),mobile phase:acetonitrile～1/12N phosphate buffer solution(70∶30,v/v),flow rate:0.8ml·min -1,detection wavelength was 345 nm,temperature of column was 40℃,external standard method was quantitative analysis method.Results:The linear relationship of serum concentration of berberine was shown well in the range of 0.72μg·L -1～578.98μg·L -1,the detectability was 0.36μg·L -1,recovery ratio was(96.06±4.74)%,degree of precision of within the day and among the day were 1.16%～2.18%、0.99%～3.04% respectively.Conclusion:This method was shown with good accuracy、good sensitivity and good specificity,can provide a valuable method for serum concentration detection of berberine.