清解宁对小鼠免疫功能的影响

Effects of QingJieNing on Immune Function in Mice

目的 :研究清解宁对小鼠免疫功能的影响。方法 :用绵羊红细胞 (SRBC)免疫小鼠 ,分别用小鼠脚掌厚度测量法观察迟发性超敏反应 (DTH)、溶血分光光度法观察体外抗体形成细胞数 (PFC)、微量血凝法观察溶血素、MTT法观察脾T、B淋巴细胞的增殖功能 ,腹腔MΦ吞噬中性红比色法观察腹腔MΦ吞噬中性红的能力。结果 :清解宁能明显抑制SRBC免疫小鼠的DTH、PFC、溶血素、脾T、B淋巴细胞的增殖以及腹腔MΦ吞噬中性红的能力。结论 :清解宁对SRBC免疫小鼠的免疫功能具有明显的抑制作用。

Objective:To study the effects of QingJieNing on immune function in mice. Methods:The animal model was made by immuning mice with sheep red blood cell(SRBC), delayed type hypereneitivity(DTH) by measuring foot thickness, qutity of plaque forming cell (PFC) by hemolytic spectrophotometry, proliferation of T and B lymphocyte by MTT colorimetry, phagocytosis ability of M Φ in abdominal cavity by neural red colorimetry were observed respectively. Results:QingJieNing can siginificantly inhibits DTH, PFC, hemolysin, proliferation of T and B lymphocyte, and phagocytosis of M Φ in mice. Conclusion:QingJieNing has siginificant inhibition effect on immune function in mice immuned by SRBC.