均匀设计法优选川贝母渗滤提取工艺

Optimum Extraction Process of F.cirrhosa D.Don.by Uniformity Design

目的 :筛选川贝母渗滤提取最佳工艺条件。方法 :采用均匀设计安排试验 ,以浸膏得率和西贝碱含量为考察指标 ,数据经多元回归处理 ,建立西贝碱的定量评价方法。对川贝母渗漉提取工艺中影响效果的乙醇浓度、乙醇用量、浸润时间三因素及条件进行优化 ,根据回归方程并结合经验 ,优选出川贝母渗漉提取工艺的最佳实验条件。结果 :川贝母用 14倍量 6 5 %乙醇渗漉

Objective:To optimize the extraction of Sipeimine of F.cirrhosa D.Don.Methods:Taking content of Sipeimine as parameters.The optimum extraction process was selected with the U 7(74)uniformity design.Results:The significant effects of alcohol concentration,alcohol volume and percolation time on the extraction of Sipeimine content were discovered.Conclusion:The optimum rhubarb percolation condition obtained was:adding 14-fold times of 65% alcohol and percolating for 7 hours.Sipeimine content ≥14.16%.