HIV-2跨膜蛋白gp36的截短及表达

Truncation and Expression of HTV-2 Trans-membrane Protein gp36

目的 将HIV-2跨膜蛋白gp36进行截短,并在大肠杆菌中表达。方法 用PCR将gp36的编码基因进行截短,回收的PCR产物纯化后克隆到连接载体pGEM-T上,然后用 BamH Ⅰ和EcoR Ⅰ切下目的基因,并构建到表达载体pGEX-4T3上,导入宿主细胞BL21(DE3),用IPTG诱导表达。结果 成功地对gp36进行了截短,并且构建到表达载体上进行表达。结论 截短的HIV-Ⅱ跨膜蛋白gp36能直接在大肠杆菌内进行表达,为跨膜蛋白的进一步应用打下基础。

Objective To truncate HIV-2 trans-membrane protein gp36 and express in E. coll. Methods Truncate the gene encoding gp36 by PCR, harvest PCR products, purify and clone into vector pGEM-T. Digest goal gene with BamH I and EcoR I, insert into expression vector pGEX-4T3, transform to E. coli BL21(DE3) and express under induction of IPTG. Results gp36 protein was successfully truncated and directly expressed in E. coli. Conclusion The study laid a foundation of further application of trans-membrane protein.