口腔鳞状细胞癌脂肪酸代谢的初步研究

A pilot study on fatty acid metabolism in oral squamous cell carcinoma

目的观察舌鳞状细胞癌细胞株TCA-83脂肪酸代谢特点,探讨利用脂肪酸合成途径进行抗癌治疗的可能性.方法采用放射性同位素标记方法和液体闪烁测量方法观察TCA-83细胞和正常人牙龈成纤维细胞[3H]掺入细胞总脂质的量,通过MTT法观察脂肪酸合酶抑制剂-浅蓝菌素对TCA-83细胞的细胞毒性作用.结果①TCA-83细胞和正常人牙龈成纤维细胞[3H]掺入细胞总脂质的量分别为68±12.7cpm/2×105细胞和13±4.2cpm/2×105细胞,差异有显著性(P=0.011).②浅蓝菌素在浓度为2.5μg/ml时,对正常人牙龈成纤维细胞的细胞毒性较小,但对TCA-83细胞仍有明显的毒性.结论脂肪酸合成活性增高是口腔鳞状细胞癌重要的生化特征,浅蓝菌素对口腔鳞状细胞癌有选择性的细胞毒作用.本研究为通过脂肪酸合成途径来控制肿瘤细胞生长提供了参考.

Objective Illuminating the fatty acid synthesis characteristic of the squamous cell (TCA - 83) carcinoma cell and to assess the possibility of exploiting the route of the fatty acid synthesis for anticancer therapy. Methods By radioactive isotope labeling and liquid scintillation counting, the [3H] acetate incorporation into total lipids in TCA-83 cells and normal gingival fibroblast was detected. Using the MTT method, we investigated the cytotoxicity characteristic of cerulenin- a specific, potent, noncompetitive inhibitor of fatty acid synthase (FAS)- to the squamous cell carcinoma cell. Results It was found that the [3H] acetate incorporation into total lipids was 68±12. 7cpm/2 ×105 cell in TCA-83 cells, while only 13 ± 4.2cpm/2×105cells in normal gingival fibroblast ( P =0.011),and found that cerulenin inhibited the TCA - 83 cell growth in dose- dependent manner even at the concentration of 2. 5 fig/ml, at which normal gingival fibroblasts were not inhibited. Conclusion Our studies demonstrate that the improving of fatty acid syntheses is a most biochemical characteristic of the squamous cell carcinoma, cerulenin exerts selective cytotoxic effects on the squamous cell carcinoma.