蛋白质组学研究方法及其在生物医学中的应用

Methodology and application of proteomics in biomedicine

蛋白质组学方法发展迅速 ,并日见成熟。蛋白芯片技术、双向电泳 -质谱技术、多维液相色谱 -质谱技术等 ,已被广泛应用于寻找疾病相关的差异表达蛋白质、疾病相关的生物标记分子以及药物靶点用于临床诊断、药物设计、以及疾病发病机理的研究等。本文对上述研究进展作了简要综述。

With the fast development of proteomics, several key techniques including proteinchip technology, two dimensional gel electrophoresis combined with mass spectrometry, multidimensional liquid chromatography coupled with mass spectrometry have been widely applied to the studies of differentially expressed proteins, disease related biomarkers for clinical diagnosis, drug targets for drug design and discovery, and even to the study of disease mechanism. The progress of proteomics and the application of methodology in biomedicine were reviewed in this article.