TaqMan-分子灯标:一种新型的荧光基因检测探针

TaqMan-Molecular Beacon: A New Type of Fluorogenic Probe for Target Gene Detection

在TaqMan及分子灯标的基础上开发了一类新型的均相荧光检测探针———TaqMan 分子灯标 (TaqMan MB) ,该探针集合了分子灯标的发夹结构及TaqMan探针降解作用的工作原理 ,使检测效果更好 .与实时PCR仪联用 ,可用于靶基因的定量检测 .

A novel homogenous fluorogenic probe, TaqMan-molecular beacon (TaqMan-MB) has been developed from TaqMan and molecular beacons, which combines the hairpin structure of molecular beacons and the cleavage mechanism of TaqMan probes and has much better detection effects than TaqMan or molecular beacons alone. Combing with a real-time PCR instnunent, TaqMan-MB presents a promising approach for quantitation of target genes.