山羊草Aegilops kotschyi及其供体种Ae.longissima和Ae.umbellulata的醇溶蛋白研究

Gliadin Variations in Aegilops kotschyi and its Progentiors Ae. longissima and Ae. umbellulata

采用酸性聚丙烯酰胺凝胶电泳(APAGE)法对11份Aegilops kotschyi及其S^1染色体组供体种Ae.longissima2份和U染色体组供体种Ae.umbellulata6份进行了醇溶蛋白位点的研究。结果表明:11份Ae.kotschyi共分离出32条带,31条具有多态性,占96.88％,每份材料可以分离出10～17条谱带,其中仅1条(3.12％)是共有带;11份Ae.kotschyi的遗传距离的变异范围在0～0.704之间,平均为0.409;11份Ae.kotschyi分离出的多数醇溶蛋白谱带均与其染色体组供体种Ae.longissi-ma及Ae.umbellulata相同,但仍有8条谱带未在两供体种中找到;11份Ae.kotschyi的醇溶蛋白多态性(96.88％)明显高于Ae.longissima(52.94％)与Ae.umbellulata(88.89％);11份Ae.kotschyi中有4份表现出了一定的特征带,分析知可能在γ区发生了较大的变异。

Using acid polyacrylamide gel eletrophoresis(APAGE), the gliadin variation of 11 Ae. kotschyi accessions and its progentiors, 2 Ae. longissima accessions and 6 Ae. umbellulata accessions were analyzed. Thirty-two gliadin bands were separated by electrophoresis and thirty-one out of them showed polymorphism (96.88%). Each material had 10 to 17 bands and only 1 band was involved in all of the 11 Ae. kotschyi accessions . The genetic distance (GD) of 11 Ae. kotschyi accessions based on gliadin ranged from 0 to 0. 704, with an average of 0.409. The patterns separated in the 11 Ae. kotschyi accessions were mostly derived from Ae. longissima or Ae. umbellulata , though 8 bands weren't found in the two progentiors. Polymophism of Ae. kotschyi (96.88 % ) was obvious higher than Ae. longissima (52. 94 % ) or Ae. umbellulata (88. 89 % ); 4 out of the 11 Ae. kotschyi accessions had specitie bands. It probably indicated that the r region in Ae. kotschyi had been changed in comparison with that of Ae. longissima or Ae. umbellulata .