用于有机磷农药残留快速检测的固定化小麦酯酶研究

Rapid Assay Study on Dichlocvos Residue by lmmobilized Wheaf Estecase Extract

以大孔径强碱性阴离子交换树脂作为固定化载体,采用离子结合法,对植物粗酶液进行固定化。在室温及酶液对载体比例为4(ml):1(g)的条件下,对酶固定化的环境pH值、缓冲液浓度及固定化特性进行了研究。得到的固定化酶对10-7mol/L水平下敌敌畏的抑制仍有明显的响应,这一效果明显优于游离酶的抑制响应。

Extracted crude wheat esterase was immobilized by ion combination method. The carrier of the immobilized enzymewas a strong basic anion exchanging resin. The effects of pH value, concentration of enzyme extracting buffer and the esteraseactivity of enzyme solution on the immobilization, as well as the kinetic characteristics of the immobilization have been investigatedunder room temperature and a fixed ratio of enzyme solution to the carrier was established. The imobilized enzyme has beenremarkably inhibited even by the level of 10-7mol/L of dichlorvos. The inhibitory effect was superior to the result of free enzyme.