四磺基酞菁铁模拟酶酶联放大镧系螯合物偶合发光体系测定过氧化氢的研究

Application of a Lanthanide Fluorescence System Coupled with the Oxidation Reaction of p-HBA Catalyzed by FeTSPc to the Determination of H_2O_2

用四磺基酞菁铁(FeTSPc)催化H2O2氧化对羟基苯甲酸(p HBA)形成弱荧光的二聚产物———联二对 羟基苯甲酸,该产物在碱性条件下能与铽、EDTA形成三元配合物,产生较强的分子内敏化发光,表现出铽离子的特征荧光发射.据此,建立了雨水中过氧化氢和血清中葡萄糖的灵敏测定方法.在最佳条件下,过氧化氢的线性范围为0～2.0×10-5mol/L,检测限为2.5×10-8mol/L.

The oxidation product of phydroxybenzoic acid (pHBA) oxidated by hydrogen peroxide in the presence of iron(III)tetrasulfonatophthalocyanine (FeTSPc) forms a ternary fluorescent complex with Tb3+ in the alkaline medium. Based on this fact, a novel lanthanide fluorescence system with a coupling reaction was developed for the determination of hydrogen peroxide in rainwater and glucose in human serum. Under optimum conditions, the calibration graph was linear over the range of 0～2.0× 10-5 mol/L. The detection limit of this method was 2.5×10-8 mol/L for hydrogen peroxide, and the standard deviation (n= 9) was 3.13 % for the determination of 1.0×10-6 mol/L hydrogen peroxide. This method can be applied to the determination of biochemical substances which produce hydrogen peroxide in oxidation reactions catalyzed by their oxidases. The feasibility in practical clinical analysis has been proven by the determination of glucose in human serum.