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定期检测肾移植患者尿中供者细胞DNA的临床意义 被引量:3

Clinical value to regularly detect donor DNA in urine of renal transplant recipients
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摘要 目的 探讨肾移植患者尿中供者细胞的出现与急性排斥反应的相关关系及其临床意义。方法 以供者为男性、受者为女性或HLA DR抗原有错配的 80例肾移植患者为研究对象 ,定期收集尿液标本 ,从中提取DNA ,利用聚合酶链反应及序列特异性引物 聚合酶链反应分别检测Y染色体上特异的基因片段DYZ 1和HLA DR抗原的基因DRB1。结果 手术当天受者的尿中即有供者细胞出现 ,随着时间的推移 ,尿中供者细胞DNA的基因表达强度逐渐减弱 ,直至术后 30d ,仍有 90 .0 %患者的尿中有供者细胞DNA的基因表达 ,其中 8例 (2 9.6 % )发生了急性排斥反应 ;出院后发生急性排斥反应者 ,90 .0 %的尿液标本中能检测到供者细胞DNA ,抗排斥治疗结束后 2周 ,83.3%仍为阳性 ,治疗过程中尿中供者细胞DNA的基因表达强度逐渐减弱 ,直至 3个月后 ,88.9%转为阴性 ;肾功能良好的稳定期患者 ,仅 6 .7%的患者尿中DYZ 1或HLA DRB1基因阳性。结论 肾移植患者尿中供者细胞DNA的检测可以作为诊断急性排斥反应 ,并与药物性肾功能损伤进行鉴别的一种方法 。 Objective To investigate the relationship between the appearance of donor cells in urine and acute rejection and the clinical implication. Methods Eighty renal transplantation patients were observed, in which the donors were male and the recipients were female, or HLA DR antigen were mismatched (30 cases were at perioperative period, 20 cases were subjected to acute rejection, 30 cases had stable renal function). Urine samples were collected regularly. PCR and PCR SSP were applied to detect DYZ 1 (special gene fragment of Y chromosome) and DRB 1of HLA DR antigen respectively after DNA were obtained.Results Perioperative period group: donor cells in urine were detected in all the patients 24?h after operation. With the development of disease, the intensity of donor DNA expression in urine was decreased generally. 30 days later, donor cells in urine disappeared only in 3 cases of 30 cases, and acute rejection happened in 8 cases of the rest 27 cases. Acute rejection group: donor cells in urine were detected in 18 cases (90%); 2 weeks following anti rejection therapy, donor cells in urine were negative only in 3 cases, still positive in the other 15 cases, and the intensity of donor DNA expression in urine was decreased generally during the treatment. Donor cells in urine were negative in 16 cases ( 88.9% ) after treatment for 3 months. Stable renal function group: DYZ 1 or HLA DRB1 was positive in 2 cases ( 6.7% ), negative in 28 cases ( 93.3% ). Conclusion PCR and PCR SSP were used to detect DNA of donor cells in urine, which would be a new method to diagnose acute rejection of renal transplantation, but would not exactly fit for those happened in early stage. The intensity change of donor DNA expression in urine represented the recovery of renal transplantation, which provided the possibility to evaluate renal allograft rejection quantitatively at the same time.
出处 《中华器官移植杂志》 CAS CSCD 北大核心 2003年第3期133-135,共3页 Chinese Journal of Organ Transplantation
基金 国家自然科学基金资助项目 ( 30 2 712 96 )
关键词 肾移植 急性排斥反应 供者细胞 DNA检测 尿液检测 Kidney transplantation Graft rejection Tissue donor DNA Gene expression Urinalysis
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参考文献7

  • 1Marsh SGE. HLA class II region sequencers. Tissue Antigens,1998, 51 :467-507.
  • 2Zhang Z, Ohkohchi N, Sakurada M, et al. Diagnosis of acute rejection by analysis of urinary DNA of donor origin in renal transplant recipients. Transplant Proc, 2001, 33:380-381.
  • 3Oba S, Tomo H, Tanabe A, et al. Expression of major histocompatibility complex(MHC) classll antigen in transplanted in kidney.Jay J Transplant, 1988, 23:359-364.
  • 4Zhang Z, Ohkohchi N, Sakurada M, et al. PCR and PCR-SSP for detection of urinary donor-origin DNA in renal transplant recipients with acute rejection. Jay J Transplant, 2001, 36: l-8.
  • 5Starzl TE, Demetris AJ, Murase N, et al. Donor cell chimerism permitted by immunosuppressive drugs: a new view of organ transplantation. Immunol Today, 1993, 14:325-327.
  • 6Lowry RP, Takeuchi T. The Thl, Th2, paradigm and transplantation tolerance: cellular and molecular mechanism. In: Alexander JW, Good RA, eds. Transplantation tolerance induction. Austin:TX R.G. Landes, 1996. 91-96.
  • 7Masri MA. Logical approach to transplant patient monitoring.Transplant Proc, 1999, 31: 3353-3355.

同被引文献37

  • 1田亚平,谷峰,郭广宏.肾移植术后血清细胞因子及生化指标的动态变化研究[J].中华检验医学杂志,2005,28(8):836-839. 被引量:8
  • 2程东瑞,王金泉.移植物功能延迟恢复[J].肾脏病与透析肾移植杂志,2005,14(5):460-464. 被引量:5
  • 3中华人民共和国国务院医疗机构管理条例.1994-09-01.
  • 4Oba S,Tomo H,Tanabe A,et al.Expression of major histocompatibility complex(MHC) classⅡantigen in transplanted in kidney.Jay J Transplant.1988;23:359-364.
  • 5Zhang J,Tong KL.Philip KT,et al.Presence of Donor and Recipient derived DNA in Cell free Urine Samples of Renal Transplantation Recipients:Urinary DNA Chimerism.Clinical Chemistry.1999;45(10):1741.
  • 6Zhang Z,Ohkohchi N,Sakurada M,et al.PCR and PCR-SSP for detection of urinary donor-origin DNA in renal transplant recipients with acute rejection.Jay J Transplant.2001;36:1-8.
  • 7Ke LD,Chen Z,Yung WK.A reliability test of standard-based quantitative PCR:exogenous vs endogenous standards.Mol Cell Probes.2000;14(2):127-135.
  • 8Li B, Hartono C, Ding R, et al. Noninvasive diagnosis of renal - allograft rejection by measurement of messenger RNA for perforin and granzyme B in urine. N Engl J Med,2001,344 :947 -954.
  • 9Romagnani P, Lazzeri E,Lasagni L,et al. IP - 10 and Mig production by glomerular cells in human proliferative glomerulonephritis and regulation by nitric oxide. J Am Soc Nephrol,2002,13:53 -64.
  • 10El - Sawy T, Fahmy NM, Fairchild RL. Chemokines: directing leukocyte infiltration into allografts. Curr Opin Immunol,2002,14:562-568.

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