期刊文献+

急性排斥反应过程中淋巴细胞趋化因子基因表达的意义

Implication of expression of lymphotactin mRNA in allografts
原文传递
导出
摘要 目的 探讨急性排斥反应过程中淋巴细胞趋化因子 (LTN)mRNA在移植心脏局部表达的意义及环孢素A(CsA)对其的影响。方法 以SD大鼠为受者 ,Wistar大鼠为供者 ,施行异位 (腹腔 )心脏移植术 ,分为术后使用CsA组 (15mg·kg-1·d-1)和不用组 ,并设SD大鼠间的心脏移植组 (同系移植组 ) ,以正常SD大鼠为对照组。采用一步法逆转录 聚合酶链反应 (RT PCR)检测术后不同时间移植心脏局部LTNmRNA的表达。结果 正常对照组和同系移植组在各时间点均未见LTNmRNA表达 ;LTNmRNA在未用CsA组的表达变化与急性排斥反应的进程相关 ,排斥反应的早期出现LTNmRNA表达上调 ,术后 5d时达到峰值 ,而应用CsA组 ,LTNmRNA的表达峰值出现延缓 ,且明显低于未用CsA组 (P <0 .0 5 )。结论 LTNmRNA的表达上调与排斥反应过程中淋巴细胞浸润密切相关 ,可能对急性排斥反应的早期诊断有帮助 ;CsA可抑制LTN基因的表达 。 Objective To investigate the implication of lymphotactin (LTN) mRNA expression in cardiac allografts and the effect of cyclosporin A (CsA).Methods Three groups of rats underwent the heterotopic cardiac transplantation: isograft group (group A), untreated group (group B) and CsA treated group (group C). The LTN mRNA expression was detected by one step RT PCR method. Results The expression of LTN mRNA was not detected in both isografts and normal hearts. The changes of LTN mRNA expression were correlated with the process of acute allograft rejection. The peak of elevated level of the LTN mRNA expression appeared in the 5th day after transplantation and CsA could delay the peak and downregulate its expression. The peak level of LTN mRNA expression in group C was significantly lower than that in group B ( P < 0.05 ).Conclusion Upregulation of LTN mRNA expression was significantly correlated with the trafficking of lymphocytes and may be useful to diagnose the acute allograft rejection earlier. It may be one of the important mechanisms of CsA to intervene the acute rejection by downregulating the LTN mRNA expression.
出处 《中华器官移植杂志》 CAS CSCD 北大核心 2003年第3期160-162,共3页 Chinese Journal of Organ Transplantation
基金 上海市医学领先专业重点学科发展基金资助项目 ( 9830 0 5)
关键词 异位心脏移植术 急性排斥反应 淋巴细胞趋化因子 基因表达 环孢素A Graft rejection Chemokines Gene expression Heart transplantation Rats
  • 相关文献

参考文献6

  • 1顾晓,杨尚琪,唐孝达,刘永,周佩军.大鼠异位心脏移植手术成功的影响因素[J].复旦学报(医学版),2001,28(1):80-81. 被引量:4
  • 2Kennedy J, Kelner GS, Kleyensteuber S, et al. Molecular cloning and functional characterization of human lymphotactin. J Immunol,1995, 155: 203-209.
  • 3Billingham ME, Cary NR, Hammond ME, et al. A working formulation for the standardization of nomenclature in the diagnosis of heart and lung rejection. J Heart Transplant, 1990, 9:587-593.
  • 4Foxman EF, Campbell J J, Butcher EC. Multistep navigation and the combinatorial control of leukocyte chemotaxis. J Cell Bio,1997,39:1349-1360.
  • 5Hautamaa D, Merica R, Chen ZY, et al. Murine lymphotactin:gene structure, post-translational modification and inhibition of expression by CD28 costimulation. Cytokine, 1997, 9: 375-382.
  • 6Wang JD, Nonomura N, Takahara S, et al. Lymphotactin: a key regulator of lymphocyte trafficking during acute graft rejection.Immunology, 1998, 95:56-61.

二级参考文献3

  • 1[1]Ono K, Lindsey ES. Improved technique of heart transplantation in rates. J Thorac Cardiovasc Surg , 1969,57: 225
  • 2[2]Rubin Y, Skutesky E, Amihai D, et al. Improved hypotheric preservation of rat hearts by furosemide. J Thorar Cardiovasc .Surg.1995,110:523
  • 3[3]Svensson LG,Loop FD. Prevention of spinal cord ischmia in aortic surgery.In:Bergan JJ, Yao JST, eds. Arterial surgery: New diagnostic und operative techniques. New York:Grune & Stratton, 1988,237

共引文献3

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部