大鼠器官移植术后的弓形虫感染及特异性抗体的检测

Evaluation of the infection after allograft and detection of the specific antibodies of toxoplasma gondii in rats

目的 观察大鼠器官移植术后弓形虫感染发病情况及环孢素A(CsA)对感染的影响 ,并探讨该病的诊断方法。方法 以Wistar大鼠为供者 ,SD大鼠为受者 ,按供、受者术前弓形虫的感染情况分为供者隐性感染组 ( 2 0只 )和受者隐性感染组 ( 2 0只 ) ,两个组均接受异位心脏移植 ,术后每组各有 10只接受CsA治疗 ,另 10只不用。术后观察动物的存活情况、发病表现 ,监测弓形虫特异性抗体IgM、IgG滴度和弓形虫循环抗原 (CAg)的变化。 结果 移植术后供者隐性感染组的弓形虫感染死亡率、CAg阳性率、弓形虫感染发病率均高于受者隐性感染组 ;使用CsA可使弓形虫感染危险性增高 ;弓形虫特异性抗体IgG、IgM的测定结果有时受CsA的影响 ,而CAg不受CsA影响 ,且较抗体出现早。结论 CsA使用可使大鼠心脏移植后的弓形虫感染发病率升高 ;弓形虫特异性抗体IgM、IgG和循环抗原 (CAg)的测定 ,并结合临床表现 。

Objective To evaluate the relationship between the use of CsA and onset of toxoplasma gondii after organ transplantation and study the effect of pathogen acquired path on toxoplasma infectious incidence.Methods ELISA was used to detect recipient's specific CAg and antibodies (IgG, IgM) after organ transplantation of rats. Results The incidence of donor acquired T.gondii infection was higher than that of reactivation of recipient latent infection before operation. The use of CsA could increase the risk of infection by T. gondii after organ transplantation. The use of CsA influenced the detection of anti IgG,IgM sometimes but had no effect in early diagnosis of T.gondii infection through detection of CAg.Conclusion The use of CsA was the main reason leading to infection of toxoplasma gondii after organ transplantion. Detection of specific anti IgG,IgM combined with clinical symptom could be used in early diagnosis.