热缺血时间对大鼠胰岛结构和功能的影响

Impact of donor warm-ischemia time on islet transplantation

目的 探讨胰腺热缺血时间对胰岛形态和功能的影响。方法 以Lewis大鼠的胰腺为研究对象 ,分别在大鼠被处死后立即、2 5min后及 30min后以胶原酶灌注 ,然后分离、纯化胰岛 ,观察各组所得胰岛的形态、活性、胰岛当量数 (IEQ)与功能。结果 各组均可获得形态完好的胰岛 ,胰岛细胞活率在 85 %以上 ;各组IEQ的差异无显著性 ;热缺血时间 2 5min组与立即灌注组相比 ,纯化后的胰岛细胞在低糖与高糖刺激下 ,胰岛素分泌量的差异无显著性 ,而热缺血时间 30min组与立即灌注组相比 ,胰岛素的分泌能力显著降低 (P <0 .0 1)。结论 热缺血时间不超过 2 5min时 ,分离、纯化后得到的胰岛在结构与功能方面的改变不显著 ,若热缺血时间延长到 30min时 。

Objective To study the impact of warm ischemia time on the outcome of islet transplantation, determine the time limit of donor organ to gain islets with enough number and normal function, therefore enlarge the donor group.Methods Adult Lewis rats (weighing 350～500?g) were studied when warm ischemia time was prolonged to 25 min and 30 min compared with the in site perfusion group. IEQ number and function of the different groups were compared. Results There was no significant difference among the groups in the islets number both before and after islet purification ( P > 0.05 ). And there was no significant difference between the group with warm ischemia time for 25 min and that in site perfusion group ( P > 0.05 ). But there was significant difference in islet function when warm ischemia time was prolonged to 30 min compared with that in site perfusion group ( P < 0.01 ).Conclusion When the donor pancreat with warm ischemia time prolonged to 25 min were used, number and morphology as well as function of the islets were not affected. But islet function was significantly impacted when the time was prolonged to 30 min.