风湿性心脏病房颤患者I_(k1)重构的分子基础

Molecular basis of I_(k1) remodeling in patients with atrial fibrillation

目的 研究心房颤动 (AF)患者内向整流钾电流 (Ik1 )重构的分子基础。方法 将 4 6例风湿性心瓣膜病接受换瓣手术患者分为三组 ,窦性心律组 (SR组 ) 18例 ,阵发性房颤组 (PAF) 7例 ,慢性房颤组 (CAF) 2 1例。应用半定量逆转录 -聚合酶链反应 (RT- PCR)技术 ,检测心房组织 Kir2 .1m RNA表达。结果 和窦性心律组相比 ,Kir2 .1m RNA在慢性房颤组中的表达明显增加 (P<0 .0 1)。结论 风湿性心瓣膜病伴房颤患者 Kir2 .1m RNA表达水平的增加可能是 IK1 上调的分子基础。

Objective\ To investigate the molecular basis of I k1 remodeling in patients with atrial fibrillation.Methods\ Right atrial appendages were obtained from 7 patients with paroxysmal AF,21 patients with chronic AF and 18 patients matched controls in sinus rhythm.All were patients undergoing valve replacement surgery.Total RNA was isolated and reversely transcribed into cDNA.In a semi quantitative polymerase chain reaction the cDNA of interest and of glyceraldehydes 3 phosphate dehydrogenase were coamplified and separated by ethidium bromide stained gel electrophoresis.Results\ Compared with SR,mRNA levels of Kir2 1 were increased in patients with CAF(0 38±0 24,0 62±0 23, P <0 01) Conclusion\ Human atrial fibrillation induces transcriptionally mediated upregulation of I K1