食管鳞癌survivin蛋白和P63蛋白的表达及其临床意义

Expression and clinical significan ce of survivin and P63protein in esop hageal squamous carcinoma

目的研究食管鳞癌(ESC)组织中survivin蛋白和P63蛋白的表达与ESC生物学行为之间的关系。方法采用免疫组织化学(SP)方法检测65例ESC组织中survivin蛋白和P63蛋白的表达,探讨两者与ESC组织分化程度、TNM分期、淋巴结转移和预后的关系。结果(1)survivin蛋白在ESC组织中的阳性表达率为55.4%,其表达越强,组织分化程度越低(P=0.00)、TNM分期越晚(ⅡB期和Ⅲ期,P=0.00)、淋巴结转移率越高(P=0.00)、两年生存率越低(P=0.00);(2)P63蛋白在ESC组织中的阳性细胞百分数为(43.2±24.7)%,其阳性细胞百分数越高,组织分化程度越低,但阳性细胞百分数的高低与TNM分期、淋巴结转移、两年生存率无关(P均>0.05);(3)survivin蛋白呈强阳性表达的病例中,其P63蛋白阳性细胞百分数明显高于survivin呈阴性和弱阳性表达的病例(P均=0.00)。结论ESC组织survivin蛋白的表达,与ESC的分化程度、TNM分期、淋巴结转移情况和预后有密切关系,它的高表达提示肿瘤患者预后不良;而P63蛋白对ESC生物学行为的影响相对较小。

Objective To investigate the expression of sur vivin and P63protein and their clini cal significance in esophageal squamou s carcinoma (ESC).Methods The survivin and P63protein express ion was detected by immunohistochemistry in 65cases of ESC.The relationshi p between the expression of these two proteins and tumor grade,TNM sta ge,lymph node metastasis and prognosis was analyzed.Results Positive rate of survivin protein wa s 55.4%.Upregulated expression of s urvivin was strongly associated wit h poor differentiation(P=0.00),late TNM stage(P=0.00),lymphatic metastasis(P=0.00),low two-year survival rate(P=0.00).P63protein expression was found in all cases.The mean proportion of positive cells was(43.2±24.7)%,and increased with poorer differe ntiation,but no correlation was fou nd with TNM stage,lymph node metastasis and sur vival rate(P>0.05).More positive P63cells were found i n ESC cases with strong survivin protein e xpression than those with negative o r weak survivin protein expression(P=0.00).Conclusions The expression of survivin protein i s closely correlated with the differentiation,TNM stage,lymph node metastasis and survival time in ESC.Strong express ion of survivin indicates poor prognosis and P63has less effect on b iological behaviors of ESC.[