沙眼衣原体感染诱导Hela细胞分泌IL-8和IL-10

Hela cells secrete interleukin-8 and interleukin-10 response to Chlamydia Trachomatis entry

目的 :探讨沙眼衣原体 (CT)感染对上皮细胞系Hela细胞产生IL 8和IL 10能力的影响。方法 :采用细胞培养法和ELISA法 ,对感染CT的Hela细胞上清进行IL 8和IL 10检测。结果 :CT感染的Hela细胞IL 8和IL 10分泌量随感染剂量增加而增高 ;感染 2 4h后 ,IL 8和IL 10开始显著增加 ,72h达高峰 ,以后开始下降 ,虽然热灭活CT(HI CT)也能诱导IL 8和IL 10表达 ,但诱导水平显著低于感染组。结论 :CT感染能诱导Hela细胞大量分泌IL 8和IL 10 ,并且呈时间和剂量依赖性 ,此种作用可能与CT入侵、细胞内复制有关。

Objective To investigate the effect of Chlamydia trachomatis serotype K infection on productions of IL-8 and IL-10 in supernatant of cultured Hela cells.Methods The levels of IL-8 and IL-10 productions were detected by cell culture method and ELISA assay.Results The amount of IL-8 and IL-10 in the cell culture supernatant rose with the increasing doses of Chlamydia trachomatis.The concentrations of IL-8 and IL-10 began to increase obviously at 24 h,and reached their highest level at 72 h,and then decreased.Heat-inactivated Chlamydia trachomatis induced lower levels of IL-8 and IL-10 production than live bacteria in vitro.Conclusion The Chlamydia trachomatis can induce the secretion of IL-8 and IL-10 of host cells in a dose and time depenedent manner and the intracellar replication of the bacteria is required for enhancing IL-8 and IL-10 productions by the infected cells.