急性白血病血清中血管内皮生长因子的表达及临床意义

Clinical significance and expression of vascular endothelial growth factor in serum of patients with acute leukemia

目的 :探讨急性白血病患者化疗前血清中血管内皮生长因子 (VEGF)水平与白血病发生及复发之间的关系。方法 :应用酶联免疫吸附法 (ELISA)定量检测 88例急性白血病和 30例正常人血清中及白血病细胞培养上清液中VEGF含量。结果 :初发白血病及复发白血病患者化疗前血清及细胞上清液VEGF含量明显高于正常人 (均P <0 .0 1) ;血清VEGF含量与外周血幼稚细胞数及骨髓中原始细胞数呈显著正相关 (r =0 .44 6 ,0 .42 1.均P <0 .0 5 ,)。结论 :血清VEGF水平可以作为诊断和预测白血病发生、复发潜能的有效指标。一定程度上可能反映体内白血病细胞的总负荷量和疾病状态。

Objective To determine the serum vascular endothelial growth factor (VEGF ) level in patients with acute leukemia and to elucidate the relation of its level with the carcinogenesis or relapse of acute leukemia and its clinical significance. Methods The VEGF levels in the serum and leukemic cell cultured supernatants were measured by sandwich ELISA in 88 acute leukemia patients and 30 healthy individuals. Results The pre-chemotherapeutic serum and supernatant VEGF level in newly diagnosed or relapsed patients with acute leukemia were significantly higher than those in healthy subjects (P <0.01). The serum VEGF level was correlated with the count of blast cells in bone marrow or peripheral blood of patients with acute leukemia (P <0.05). Conclusion The pretherapeutic serum VEGF level of patients with acute leukemia appears to be a predictor of the carcinogenesis or relapse of acute leukemia and reflects the tomor burden of acute leukemia.