摘要
目的 :探讨参附注射液对肺表面活性物质合成 (PS)的影响。方法 :采用无血清成年大鼠肺组织培养 ,液体闪烁计数测定肺组织 [3H]胆碱掺入量 ,化学定磷法及薄层层析法测定总磷脂 (TPL)及磷脂酰胆碱 (PC)。结果 :参附注射液以剂量依赖方式促进肺组织 [3H]胆碱的掺入 ,并提高肺组织总磷脂、PC的含量及PC/TPL比值 ,该效应可被蛋白激酶C(PKC)抑制剂H7和钙调蛋白抑制剂W7阻断。人参总皂甙及单体Rb1,Rg1均可促进 [3H]胆碱的掺入。结论 :参附注射液可以促进PS的合成 ,其效应有赖于PKC及钙调蛋白的介导 ,人参皂甙是参附注射液促PS合成的有效成分之一。
Objective To explore the effect of shenfu injection on pulmonary surfactant synthesis.Methods Lung explants of adult rats were cultured in free serum DMEM. choline incorporated into lung tissue was determined by liquid scintillation system. The contents of total phospholipid (TPL) and phosphatidylcholine (PC) in lung tissue were measured by inorganic phosphorus quantitative analysis and thin-layer chromatography, respectively. Results Shenfu injection enhanced choline incorporation in a dose-dependent manner and increased the contents of TPL and PC as well as the ratio of PC/TPL .Both protein kinase C (PKC) antagonist H 7 and calmodulin antagonist W 7 inhibited the effects of Shenfu injection . The ginsenosides, ginsenoside Rb1,and ginsenoside Rg1 promoted the choline incorporation. Conclusion Shenfu injection can enhance the synthesis of pulmonary surfactant through pathways mediated by PKC and calmodulin. The ginsenoside is an effective component in Shenfu injection.
出处
《湖南医科大学学报》
CSCD
北大核心
2003年第2期137-140,共4页
Bulletin of Hunan Medical University
基金
教育部高等学校骨干教师资助计划
