用于倍性检测的对虾样品的保存方法

A PRESERVING METHOD FOR PLOIDY DETECTION OF SHRIMP SAMPLES

采用9种保存方法分2个温度梯度,对待测倍性的日本对虾幼体样品进行处理,通过对利用流式细胞仪检测的不同样品直方图进行比较,筛选出一种较为理想的用于倍性检测的对虾样品的保存方法。结果表明:新鲜样品在分散液中捣碎,加入一定量的二甲基亚砜(DMSO)后可在-20℃下保存较长时间。

Shrimp samples prepared for ploidy detection were treated with nine varieties of methods and preserved under two temperature levels for different duration. Good preservation method for shrimp ploidy detection was selected out through comparison of histograms obtained by flow cytometry. The results showed that the samples which were crushed in dispersion fluid with addition of certain amount of DMSO could be preserved for long time under- 20℃ than those using other varieties of methods.