金属离子和pH值对九孔鲍几种消化酶活力的影响

EFFECTS OF METAL IONS AND pH VALUE ON THE ACTIVITIES OF DIGESTIVE ENZYMS FROM Haliotis diversi- color supertexta

研究了金属离子对九孔鲍(Haliotis diversicolor supertepta)褐藻酸酶、琼脂酶及纤维素酶活力的影响。结果表明,3种酶的最适 pH不同。分别为褐藻酸酶 8.8、琼脂酶 4.2、纤维素酶4.5～5.0。MgSO_4为褐藻酸酶的激活剂、BaCl_2为琼脂酶的激活剂、MnCl_2是纤维素酶的激活剂。

In this paper, the effects of metal ions and pH value on the enzymic activity of algalase, agarase and cellulase of the abalone Haliotis diversicolor supertexta were studies. The results showed that Mg2 + is the activator for the enzyme algalase; Ba2 + that for the enzyme agarase; and Ba2+ and Mn2 + are that for the enzyme celluase. The studies also revealled that the enzyme agalase activity is very sharply inhibitted by the metal ions such as Cu2 + , Pb2+ , Ag+ , Zn2 + and Fe3+ .