强骨抗萎方对模拟失重大鼠骨代谢影响的观察

Effects of "Qiang Gu Kang Wei" Compound Prescription on Bone Metabolism in Rats under Simulated Weightlessness.

目的探讨中药复方“强骨抗萎方”对模拟失重大鼠骨代谢的影响。方法SD大鼠随机分成 :自由活动对照组、悬吊对照组、悬吊中药大 (3 0 g/kg)、中 (2 0 g/kg)、小 (1 0 g/kg)剂量共 5组。后 3组给予中药灌胃“强骨抗萎方”(1ml/kg) ,实验期 2 1d。观察“强骨抗萎方”对大鼠骨生物力学、矿盐含量、骨密度、骨钙素 (BGP)的作用。结果悬吊大鼠中药组骨生物力学性能各指标、骨密度、骨矿含量均有不同程度的增加 ,血清BGP含量增高 ,骨组织BGP含量有增高趋势。结论“强骨抗萎方”可以有效改善失重状态骨的生物力学特性 ,增强骨密度 ,促进骨矿盐的沉积 ,减少骨丢失。

Objective To investgate the effect of 'Qiang Gu Kang Wei' prescription on bone metabolism in simulated weightless rats. Method Male SD rats were randomly divided into five groups :1) free active control(FAC);2) tail suspended control(TS);3) tail suspended and treated with large dose of the prescription(TS+L)(30 g/kg); 4)tail suspended and treated with middle dose of the prescription(TS+M)(20 g/kg);5) tail suspended and treated with small dose of the prescription(TS+S)(10 g/kg).The experiment lasted 3 weeks.The effect of the prescription on bone biomechanical properties,bone density, bone mineral contents, and bone biochemical markers of bone metabolism were examined .Result Bone biomechanical properties,bone density,total and bound bone gla protein(BGP) of serum all incerased significantly in three groups treated with the prescription. Conclusion 'Qiang Gu Kang Wei' prescription prevents bone loss,hence maintains bone density and bone biomechanical properties under simulated weightlessness.