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LIF,VEGF,CD57和CD68在大鼠胚胎移入小鼠子宫后的表达 被引量：3

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摘要 种间妊娠失败是异种克隆的主要障碍.为探讨种间妊娠失败的原因,将大鼠囊胚移入假孕第3天的小鼠子宫内.以前的研究显示,大鼠胚胎只能在小鼠子宫内发育到第9天.本实验结果表明,异种胚胎移植后母胎界面CD57和CD68分子表达较同种移植的增强.免疫组织化学和免疫印迹研究均显示,异种胚胎移植后白血病抑制因子(LIF)表达增强,但血管内皮生长因子(VEGF)表达下降.在体外共培养系统中,大鼠外胎盘锥在小鼠子宫蜕膜细胞上黏附率、扩展率和扩展面积较同种的显著降低.这些结果提示,大、小鼠种间妊娠中免疫排斥反应增强,异种胚胎侵入能力降低,可能是导致大、小鼠种间妊娠失败的重要原因.
作者 戴博杰 曹宇静 王希朝 周家喜 张键 陈大元 段恩奎
机构地区 中国科学院动物研究所计划生育生殖生物学国家重点实验室
出处 《科学通报》 EI CAS CSCD 北大核心 2003年第9期930-935,共6页 Chinese Science Bulletin
关键词 异种克隆 种间妊娠 胚胎移植 LIF VEGF CD57 CD68 大鼠 小鼠 基因表达
分类号 Q78 [生物学—分子生物学]
  • 相关文献

参考文献24

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共引文献12

同被引文献41

  • 1王希朝,段恩奎.金色中仓鼠胚胎植入小鼠子宫后PTEN、FAK、Shc信号分子的异常表达(英文)[J].动物学报,2005,51(6):1067-1072. 被引量：1
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科学通报
2003年 第9期

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