摘要
分别以猪、牛、马等红血球超氧化物歧化酶(简称SOD)为研究对象,探讨它们在各种温度、pH、有机物和无机盐溶液中的活性变化。实验表明,不同来源的SOD对温度都有较大的耐受性;在pH 4～12范围内,SOD相对酶活性能保持50%以上;用还原剂处理SOD对其活性影响较大,用过氧化氢处理牛血SOD,其聚丙烯酰胺凝胶电泳图谱酶活力条带明显减少。
Erythrocytes SOD prepared from forcine, bovine and horse were studied. A regulation in compliance with activity change under various temperature, pH, organic and inorganic salt solution is given. It is revealed that SOD_s of different resources share the same high resistance against temperature. In pH range 4～12, SOD activity can reach as high as 50%. After treated with reducing agent, SOD showed many changes in its activity. An obvious reduction in bovine SOD treated with H_2O_2 and undergone polyacrylamide gel electrophoresis activity belt, were observed.
关键词
理化性质
活性
歧化酶
红细胞
erythrocytes
dismutase
characteristics
physical and chemical characteristics
activity comparision
