血灵合剂对癌症化疗白细胞影响的动物实验和临床观察

Animal Experiment and Clinical Observation to the Effects of Xuelinheji on Leukocyte of Cancer Chemotherapy

目的 :观察院制血灵合剂对荷瘤小鼠白细胞的影响及对癌症患者化疗后白细胞的影响。方法 :动物实验 ,NIH小鼠造模成骨髓抑制模型50只 ,随机分成5组 ,分别给以NS、CTX、CTX +血灵合剂、CTX+大量血灵合剂、CTX +沙肝醇利血生。荷S180NIH小鼠50只随机分为5组。分别给以NS、CTX、CTX+血灵合剂、CTX +大剂量血灵、血灵合剂 ,于瘤龄3、5、7、9、12天采尾静脉血 ,查血象 ,统计学处理。临床观察WBC低于4×109/L的升白作用 ;与化疗同时应用观察对骨髓的影响。结果 :骨髓抑制造模小鼠WBC第3～12天在2 4×109/L～2 7×109/L之间 ,加用血灵合剂时为3 09×109/L～9 57×109/L。荷瘤小鼠用CTX组WBC为3 54×109/L～2.64×109/L之间 ,用CTX+血灵合剂常规组为6 15×109/L～6 10×109/L,用CTX+大剂量血灵合剂组为6 37×109/L～10 47×109/L。统计学处理有显著意义。临床观察结果证实血灵合剂能缩短血象恢复时间 ,与化疗同时应用有保护骨髓的作用。结论 :血灵合剂有较好的升白作用 。

Objective:Observe the effects of Xuelinheji made by our college on mice's leukocyte and the leukocyte of cancer patients after chemotherapy.Methods:Animal experiment,make NIH mice into marrow inhibition model,divide 50 NIH mice into 5 groups randomly,respectively give them NS,CTX,CTX+Xuelinheji,CTX+more Xuelinheji and CTX+Shaganchun Lixuesheng.Divide randomly 50 S180 NIH mice into 5 groups,respectively give them NS,CTX,CTX+Xuelinheji,CTX+more Xuelinheji and Xuelinheji,take the blood from the tail vein at the 3rd,5th,7th,9th and 12th day of tumor,test the hemogram and make statistics.Clinically observe the WBC lower than 4×109/L,observe the effect on marrow while applying chemotherapy.Results:For the marrow inhibition model mice,the WBC is 2.4×109/L～2.7×109/L at the 3～12th day,3.09×109/L～9.57×109/L added with Xuelinheji.For S180 NIH mice of CTX group,WBC is 3.54×109/L～2.64×109/L;6.15×109/L～6.10×109/L for the normal group of CTX+Xuelinheji,6.37×109/L～10.47×109/L for CTX+more Xuelinheji.There's obvious meaning in statistical measurement,P<0.05.Results:The clinical observation proves Xuelinheji can shorten the recovery time of hemogram and protect the marrow applied with chemotherapy.Conclusion:Xuelinheji can better increase leukocyte and protect the marrow in chemotherapy.