摘要
采用不同培养基对西瓜(CitrullusVulgaris)的子叶、下胚轴和茎尖进行分化培养.结果表明:以MS为基本培养基,附加BA0.4mg/L处理时,3d苗龄的子叶的分化频率最高,可达95.5%,12d左右可见不定芽出现.西瓜对卡那霉素(Km)比较敏感,当培养基中添加20mg/L的Km即能完全抑制不定芽和不定根的分化.
The regeneration frequency of different explants of Citrullus Vulgaris (cotyledon,hypocotyl and terminal bud) on the different medium have been studied.The results showed that the cotyledon cultured for 3 days has the highest regeneration frequency of 95.5%,when it was placed in the MS basic medium supplemented with BA0.4mg/L.Furthermore,adventitious bud was appeared after 12 days culture.The Citrullus Vulgaris is more sensitive to Km,the differentiation of adventitious bud and adventitious root were restricted completely on the MS basic medium supplemented with Km 20mg/L.
出处
《广西师范大学学报（自然科学版）》
CAS
2003年第2期75-78,共4页
Journal of Guangxi Normal University:Natural Science Edition
基金
广西科技攻关项目(桂科攻0228019-2)
