急性坏死性胰腺炎胰外器官微循环改变及乌司他丁的影响

Effect of Urinastatin on Microcirculation of Extrapancreatic Organs in Rats with Acute Necrotizing Pancreatitis

目的 探讨急性坏死性胰腺炎 (ANP)时肺、肾、肠微循环的变化及乌司他丁 (UTI)的保护作用。方法 SD大鼠 48只 ,随机均分为对照组、ANP组及UTI组。ANP组、UTI组于胰被膜下均匀注射 5 %牛磺胆酸钠制成ANP模型 ,对照组则注射等量生理盐水。UTI组于制模后即由股静脉缓慢注射UTI 10 0 0 0u/kg ,对照组及ANP组则注射等量生理盐水。分别于制模后 2h及 6h采用放射性生物微球法测定肺、肾及末端回肠组织的血流量变化。结果 与对照组相比 ,ANP组制模后 2h及 6h肺、肾、肠组织血流量显著减少 (P<0 .0 5 ) ;与ANP组相比 ,UTI组 2h及 6h各组织血流量均有明显增加 (P<0 .0 5 )。结论 微循环障碍是ANP胰外器官损害的重要原因之一 ,UTI对ANP时胰外器官微循环障碍有明显的保护作用。

Objective To explore the effects of urinastatin(UTI) on microcirculation of extra pancreatic organs in rats with acute necrotizing pancreatitis(ANP). Methods A total of 48 rats were randomized into control group, ANP group and UTI group. The model of ANP was established by uniform injection of 5% sodium taurocholate solution under pancreatic capsule, only injection of normal saline in control group. Then the rats of UTI group were injected with UTI through the femoral vein, the rats of ANP group and control group were injected with normal saline. The blood flow of lung, kidney and distal small intestine was measured by radioactive biomicrosphere technique at 2 h and 6 h after ANP.Results Compared with the control group, the blood flow of lung, kidney and intestine was decreased significantly in the ANP group at the 2 h and 6 h after ANP ( P <0.05), compared with the ANP group, the blood flow was increased significantly in UTI group ( P <0.05). Conclusion Microcirculation disorder is an important factor of the extrapancreatic organ damage in ANP, and UTI plays a protective role against microcirculation disorder of the extrapancreatic organ in ANP.