肝细胞生长因子对大鼠移植小肠粘膜结构的保护作用

The Protective Effect of Hepatocyte Growth Factor on Grafted Structure after Small Bowel Transplantation in Rat

目的 探讨肝细胞生长因子 (HGF)对大鼠移植小肠粘膜结构的保护作用。方法 近交系Wistar (RT1k)大鼠行异位全小肠移植后第 2天开始给予肠外营养至第 10天 ,对照组 (n =10 )行常规TPN支持 ,HGF组 (n =10 )行常规TPN支持的同时加用人胎肝细胞生长因子 15 0 μg/ (kg·d) ,观察移植肠粘膜结构的形态学参数如绒毛高度、绒毛宽度、隐窝深度、粘膜厚度及绒毛表面积的变化 ,以及移植肠上皮细胞超微结构变化及移植肠粘膜蛋白质和DNA含量的改变。结果 移植前两组肠粘膜形态学参数差异无显著性意义 ,行移植及TPN后对照组各参数较移植前明显减小 (P<0 .0 5 ) ;而HGF组各参数与移植前比较变化不明显 (P>0 .0 5 ) ,但明显高于对照组 ,分别是绒毛高度为 ( 2 98.79± 2 2 .3 1) μmvs ( 176.45± 14 .62 ) μm ,P=0 .0 0 1;绒毛宽度为 ( 10 7.46± 12 .3 4) μmvs ( 74.2 0± 16.85 ) μm ,P =0 .0 0 4;隐窝深度为 ( 10 4.5 6± 11.17) μmvs ( 74.45± 8.3 4)μm ,P =0 .0 0 0 5 ;粘膜厚度为 ( 4 0 9.5 3± 3 5 .83 ) μmvs( 2 5 9.3 8± 2 4.65 ) μm ,P =0 .0 0 3 ;绒毛表面积为 ( 0 .10 1± 0 .0 11)mm2 vs ( 0 .0 41± 0 .0 0 5 )mm2 ,P =0 .0 0 1。HGF组蛋白质含量明显高于对照组 (P =0 .0 12 )

Objective To study the protective effect of hepatocyte growth factor(HGF) on grafted mucous membrane of transplanted small bowel.Methods Total small bowel transplantation was made in inbred Wistar (RT1 k) rats heterotopically,either total parenteral nutrition (control group, n =10) or hepatocyte growth factor supplemented TPN (HGF group, n =10) was given to the recipients from the 2nd day to the 10th day postoperatively. Morphological parameters of the transplanted intestinal mucosa, such as mucosal villous height, villous width, crypt depth, mucosal thickness and villous surface area were observed. Variation of ultrastructure of transplanted epithelial cells was observed. Composition of mucosal protein and DNA content were tested. Results Comparison between HGF group and the control group were as follows. Mucosal villous height (298.79±22.31) μm vs (176.45± 14.62) μm, P =0.001, villous width (107.46± 12.34) μm vs (74.20±16.85) μm, P =0.004, crypt depth (104.56±11.17) μm vs (74.45±8.34) μm, P =0.000 5, mucosal thickness (409.53±35.83) μm vs (259.38±24.65) μm, P =0.003, and villous surface area (0.101± 0.011) mm 2 vs (0.041±0.005) mm 2, P =0.001 were found significantly increased in HGF group compared with control group, there were no obvious difference decrease as compared to pretransplant parameters.Mucosal protein composition was higher in HGF group than that in control group (89.65± 9.28) mg/g wet wt vs (53.73± 11.45) mg/g wet wt, P =0.012, baseline (92.64±10.52) mg/g wet wt, nearly equal to baseline; DNA composition was also high in HGF group (0.89±0.09) mg/g wet wt vs (0.51±0.06) mg/g wet wt, P =0.008, baseline (0.91± 0.09) mg/g wet wt. Nearly normal ultrastructure of the graft was maintained in HGF group, atrophied microvilli and broken mitochondrial crista were observed in control group.Conclusion Hepatocyte growth factor can improve mucosal structure, preserve enterocyte ultrastructure of isograft after small bowel transplantation in rat.