肝细胞肝癌中HSP90α的表达及意义

Expression of Heat Shock Protein 90α in Hepatocellular Carcinoma and Its Significance

目的 研究热休克蛋白 90α(HSP90α)在人肝细胞性肝癌 (HCC)组织中的表达 ,并探讨其与肿瘤生物学行为及预后的关系。方法 采用免疫组织化学方法检测 10例正常肝组织、40例HCC组织及其癌旁组织中HSP90α的表达。结果 HSP90α在正常肝脏、癌旁组织及肝细胞性肝癌中的表达阳性率分别为 10 .0 %、5 2 .5 %及 72 .5 % ,在肝细胞性肝癌中的表达阳性率明显高于正常肝组织和癌旁组织 (P<0 .0 5 )。HCC的HSP90α阳性表达与肿瘤的临床分期、分化程度、累及浆膜状况及淋巴结转移状况显著相关 (P<0 .0 5 ) ;而与肿瘤数目无关 (P>0 .0 5 )。HSP90α阴性表达者的预后显著优于HSP90α阳性表达者 ,术后平均无瘤生存期前者为 3 8.6个月 ,后者为 2 5 .5个月 (P<0 .0 5 )。结论 HSP90α在肝细胞性肝癌中的表达明显增强 ,HSP90α表达与HCC的临床分期、肿瘤分化程度、累及浆膜状况及淋巴结转移状况有关 ,可作为判断预后的指标之一。

Objective To investigate the expression of heat shock proteins 90α(HSP90α) in human hepatocellular carcinoma and the relationship between its expression and biologic behavior of tumor and prognosis. Methods Using the immunohistochemical SP method, HSP90α expression was detected in liver tissue from 10 normal individuals, 40 patients with hepatocellular carcinoma(HCC) and adjacent noncancerous liver tissues. Results The positive expression rate of HSP90α was 10.0%,52.5%,72.5% in normal liver tissues,adjacent noncancerous liver tissues,hepatocellular carcinous tissues respectively. A significantly higher distribution of HSP90α positive expression in HCC tissues compared with adjacent noncancerous liver tissues and normal liver tissues was obtained ( P <0.05). The positive expression of HSP90α in HCC was correlated with clinical stage, tumor differentiation, serosal condition and lymph node metastasis ( P <0.05), but not to tumor number ( P >0.05). It was also correlated with prognosis of HCC. The mean tumor free survival of patients with HSP90α negative expression was 38.6 months while that of HSP90α positive expression was 25.5 months ( P <0.05). Conclusion HSP90α is overexpressed in human hepatocellular carcinoma. HSP90α could be used as an indicator to judge the clinical stage, tumor differentiation, serosal condition, lymph node metastasis and prognosis of HCC.