摘要
目的 探讨胆囊癌的发生与细菌L型感染和 p2 1及 p5 3基因突变的关系。 方法 采用免疫组化SP法和革兰氏染色技术 ,对 40例胆囊癌及 40例慢性胆囊炎组织中的细菌L型和突变型 p5 3蛋白及p2 1蛋白进行检测 ,并对胆囊癌组织中的细菌L型阳性伴 p2 1及 p5 3蛋白阳性表达结果和细菌L型阴性伴p2 1及 p5 3蛋白阳性表达结果进行对比分析。结果 胆囊癌组织中革兰氏染色细菌L型检出率为 77.5 % ( 3 1/4 0 ) ,明显高于胆囊炎的 5 7.5 % ( 2 3 /4 0 ) ,P<0 .0 5 ,且与免疫组化细菌L型抗原表达阳性率〔80 .0 % ( 3 2 /4 0 )〕具有一致性 ;胆囊癌组织中的p2 1及 p5 3蛋白表达阳性率分别为 62 .5 % ( 2 5 /4 0 )和 65 .0 % ( 2 6/4 0 ) ,明显高于胆囊炎组织中的 p2 1及 p5 3蛋白表达阳性率〔2 .5 % ( 1/4 0 )和5 .0 %( 2 /4 0 )〕 ,P<0 .0 5 ;胆囊癌组织中的细菌L型阳性者其 p2 1及p5 3蛋白表达阳性率分别为 75 .0 % ( 2 4/3 2 )和 78.1% ( 2 5 /3 2 ) ,明显高于细菌L型阴性者的p2 1及 p5 3蛋白的表达阳性率〔12 .5 % ( 1/8) ,12 .5 % ( 1/8)〕 ,P <0 .0 5。结论 细菌L型参与了 p2 1及p5 3基因的突变 ,并在胆囊癌的发生中可能起协同作用。
Objective To study the relationship between mutation of the p53 gene, p21 gene and bacteria L form in gallbladder carcinoma. Methods Forty cases of gallbladder carcinoma and 40 cases of chronic cholecystitis were studied by using Gram staining and immunohistochemistry (SP method) to detect the positive rate of L forms antigen, p21 and p53 protein overpression. And the relationship between the expression of p21 and p53 in L form infection positive group and that in L form infection negative group was discussed. Results There was no statistical difference between the L form positive rate in patients with gallbladder carcinoma with Gram staining and immunohistochemistry ( P >0.05). The positive expression rate of p21 and p53 in gallbladder carcinoma was 62.5%(25/40) and 65.0%(26/40) respectively. The expression values of p21 and p53 in chronic cholecystitis was 2.5%(1/40) and 5.0%(2/40) respectively, which was significantly different from that of gallbladder carcinoma ( P <0.05). The expression of p21 and p53 was significantly higher in L form infection positive group than in that with L form infection negative group ( P <0.05). Conclusion Bacteria L form may be one of the direct factor leading to mutation of p53 and p21 during gallbladder oncogenesis.
出处
《中国普外基础与临床杂志》
CAS
2003年第3期246-248,252,共4页
Chinese Journal of Bases and Clinics In General Surgery
