促凋亡基因bad的表达与肝内胆管细胞癌分化的关系

The Expression of Pro Apoptosis Gene Bad in Intrahepatic Cholangiocarcinoma and Its Relationship to Differentiation of the Tumor

目的 探讨促凋亡基因bad在原发性肝内胆管细胞癌 (ICC)中的表达及其与肿瘤分化程度的关系。方法 采用免疫组织化学DakoEnvision方法 ,对 48例原发性肝内胆管细胞癌 (ICC组 )和 2 5例肝细胞肝癌的癌旁肝组织汇管区胆管上皮组织 (对照组 )中bad基因的表达情况进行检测。结果 bad基因在ICC中的表达阳性率明显高于对照组 (P<0 .0 1) ;在中、低分化的ICC中bad基因的阳性表达水平明显高于高分化的ICC(P<0 .0 5 )。结论 在低分化的胆管细胞癌中bad基因的表达明显增强 ,bad基因的表达与肿瘤的分化程度相关。

Objective To investigate the expression of pro apoptosis gene bad in intrahepatic cholangiocarcinoma (ICC) and its relationship to differentiation of the tumor.Methods The immunohistochemistry technique by Dako Envision system and rabbit anti human bad polyclonal antibodies were adopted. The expression of bad was detected in 48 cases of ICC and 25 cases of control tissues.Results Bad immunoreactivity in 48 cases of ICC was higher than that of bile duct epithelium in 25 cases of control tissues. And contrasted with 21 cases of well differentiated ICC, bad immunoreactivity was higher in 27 cases of middle and poor differentiated ICC.Conclusion The expression of bad gene may be related to the differentiation of ICC.