斯克里布纳短体线虫的单异活体培养

MONOXENIC CULTURE OF Pratylenchus scribneri ON EXCISED ROOTS OF FOUR CROPS

采用链霉紊含量1000单位/ml、青霉素含量800单位/ml和放线菌酮含量15μg/ml的混合液作线虫的表面消毒剂,用无菌条件下生长在White培养基上的玉米、大豆、小麦和高梁4种植物的离体根均成功地培养了斯克里布纳短体线虫Ptatyleuchus sctibueri。在28℃和33℃下,培养效果均好。在28℃培养9周,P.sctibueri在玉米离体根上繁殖了8.4倍。通过根和培养基块移置都获得继代培养。培养的线虫具有正常的侵染能力。

Pratylenchus scribneri was monoxenically cultured in petri dishes under aseptic conditions both at 28℃ and 33℃,using respectively excised roots of the corn,soybean,wheat and sorghum grown on White's medium.A 8-fold increase in number of nematodes was observed from P.scrilmeri cultured on excised corn roots at 28℃ for 9 weeks.For surface sterilization,females of P.scrilmeri were incubated in a solution containing streptomycin sulfate (1000 units/ml) ,penicillin (800 units/ml) and actklione (15μg/ml) for 20 minutes,followed by five rinses in sterile distilled water.