水稻耐盐机理的研究Ⅱ、不同基因型愈伤组织耐盐性比较

STUDIES ON SALT TOLERANCE MECHANISM OF RICE Ⅱ.COMPARISON OF SALT TOLERANCE OF CALLI IN DIFFERENT GENOTYPES

氯化钠胁迫对水稻愈伤组织的生长有明显的影响。表现为存活率下降、生长量减少以及干/鲜值增加等。然而用上述指标进行比较,具有植株水平耐盐性差异的不同基因型,愈伤组织之间却没有显著差异,表明植株水平的耐盐程度与愈伤组织的耐盐程度之间没有直接联系。在氯化钠胁迫的情况下,耐盐基因型愈伤组织的K^+含量低于盐敏感基因型,而前者的Na^+和Cl^-含量却略高于后者。这种趋势与植株水平不一致,暗示了愈伤组织水平对无机离子的吸收可能具有与植株水平不同的机制。

Sodium chloride stress had a great impact on the growth of rice calli which was reflected by the decrease in survival ratio and relative growth and increase in dry/fresh ratio of the calli with increasing sodium chloide concentrations.No significant differences,however,were found among calli of different genotypes that markedly differed in salt tolerance at the whole plant level,indicating that there is no direct relationship between salt tolerance at the callus level and at whole plant level.Under sodirm chloride stress,the K+content in the calli of the salt tolerance genotypes was lower than that of the salt sensitive genotypes ,but the former had higher Na+and Cl-contents than the latter.This pattern was different from the whole plant level,implying that different mechanisms of ion absorption might be involved at the callus level.