柞蚕抗菌肽对水稻白叶枯病菌的抑制作用

THE INHIBITORY EFFECT OF ANTIBACTERIAL PEPTIDE FROM CHINESEOAK SILKWORM, ANTHERAEA PERNYI ON THE LEAF BACTERIAL BLIGHTXANTHOMONAS COMPESTRIS PV ORYZAE

柞蚕蛹诱导含抗菌肽的免疫血淋巴,对水稻白叶枯病病原细菌(X(?)(?)pv (?))有明显的抑菌作用,对中国白叶枯病菌Ⅳ群强致病力菌株(RX77)的抑菌圈直径达12mm。电镜观察抗菌肽作用于白叶枯病菌能使其细胞膜破坏,内容物渗出而致死亡。处理10～30min即有效果。将抗菌肽与白叶枯病菌按不同比例混合后接种于水稻秧苗及孕穗期的叶子上,20天后调查病斑平均长度,经方差分析认为抗菌肽对病菌致病力有显著的减弱作用。

Some inducible antibacterial peptides were developed from the immunized haemolymph of Chinese oak silkworm ,Autheroca pernyi by injecting with Escherichia coll.Antibacterial peptid hed a strong inhibitory effect on the pathogen of rice leaf bacterial blight disease,Xanthomomas compestris pv aryzaeObservated the ultra structure of X.Oryzoe treated with antibacterial peptide by electromicroscope.The results showed the primary target of antibacterial peptide was against the membrane of cells,after 10-30 min the cell wall was be-stroied and the intercellular substances were pressed out of cell subsequently.Different ratio (1:10-200) mixture between the immunized haemolymph;suspension of X.oryzae(106cell/ml) were used to infect the leave of seedling or booting stage of rice.After 20 days,investigated the average lenght of leaf spot infection and counted with variance analysis.The results showed antibacterial peptide from immunized haemolymph had a distinct infectivity effect on the X.oryzae