马桑内酯致大鼠点燃效应癫痫间期蓝斑去甲肾上腺素荧光组织化学的研究

Noradrenaline Fluorescence Histochemical Study on the Locus Coeruleus of Kindling Rat Induced by Coriaria Lactone

用去甲肾上腺素(NA)荧光组化法研究马桑内酯致大鼠点燃效应癫痫发作间期的蓝斑,荧光强度通过显微摄影的自动曝光系统进行相对定量。结果显示:点燃效应癫痫发作间期蓝斑NA比对照组减少,作者认为NA的抑制作用减弱是导致癫痫的原因之一。

Fourteen male Wistar ratswere divided into two groups. The experi-mental rats were injected with subconvul-sive dosage of coriaria lactone (1 mg/kg)intramuscularly per 3.5 days. The controlswere injected with normal saline, After 26injections, the loci coeruleus of kindlingrats were studied with the noradrenaline(NA) fluorescence histochemical techniqueat the time between seizures. The NA flu-orescence could be clearly visulized underfluorescent microscope. The intensity offluorescence was reflected by autoexposure-meter of the fluorescent microscope. Thebrighter the fluorescence, the shorter theautoexposure time. The intensity of NA fluo-rescence in the locus coeruleus of exper-imentel animals was weaker than that ofthe controls. Since NA plays an inhibitoryrole in cerebral cortex,the decrease of NA,either induced by repeated injections ofcoriaria lactone or due to the time of sam-ple taken after seizure,needs further study.