癫痫持续状态大鼠的蓝斑组织化学与图像分析研究

Histochemical and Image--Analytic Study of the Rat Locus Coeruleus during Status Epilepticus

作者用马桑内酯致大鼠癫痫持续状态,取蓝斑用荧光组织化学方法显示去甲肾上腺素(NA),并用显微摄影自动曝光时间和图像分析两种方法测定荧光强度。结果:均显示大脑皮质包埋马桑内酯微粒的大鼠癫痫持续发作6小时后,蓝斑中NA荧光增强。用单胺氧化酶组化和图像分析法观察到该处酶活性增强。观察到在癫痫持续状态时NA起了一定的调控作用。

Twenty-four adult maleWistar rats were used in this study. Statusepilepticus was provoked in 10 rats byembedding coriaria lactone particle intothe left cerebral motor cortex. In thecontrols were embedded particles withoutcoriaria lactone. After 6 h of continuousseizure, the locus coeruleus was studiedwith the noradrenaline (NA) fluorescencehistochemical technique and enzyme histo-chemical test for monoamine oxidase(MAO). The intensity of NA fluorescencewas detected with fluorescent microscopeautoexposuremeter and analysed withMIAS-200 Image Analyser. The studygroup showed a parallel increase of NAfluorescence as compared with that of thecontrol group by both measurements. NAplays an inhibitory role in the cortex. Ourdata suggest that the increase of NA inlocus coeruleus may be due to the reduc-tion of NA released from axon terminal.Reduction in inhibition could be one of themechanisms of seizure activity. The inten-sity of MAO was detected with MIAS-200Image Analyser. The regulation of monoa-mine metabolism by MAO in the centralnervous system and the increase of MAOactivity in the continuous seizure groupmay be induced by the accumulation of NAin the locus coeruleus soma.