大细胞性淋巴瘤的形态学与免疫组织化学——58例石蜡切片染色结果分析

The Morphology and Immunohistochemistry of Large Cell Lymphomas (A Study of 58 Cases)

用抗T细胞、B细胞,组织细胞及Ki-1抗原单克隆抗体对58例大细胞性淋巴瘤的石蜡切片进行染色,并与组织学改变加以比较。结果发现58例中B细胞性43例(74.1％),外周T细胞性15例(25.9％)。其中2例B,1例T细胞瘤形态学上为大细胞间变性,并为Ki-1阳性。外周T细胞淋巴瘤的有价值的形态改变为粉尘状染色质,中等大小的嗜酸性核仁,浅染胞浆,血管增生及嗜酸性细胞浸润。大细胞B淋巴瘤则为边集粗糙之染色质,嗜碱性核仁及浆细胞分化。

Fifty-eight ease of large cell lymphoma were studied by using a panel of monclonal antibodies reactive in routinely fixed waxembedded tissues. Forty-three of 58 cases (74.1％) were with B-cell phenotype, whereas 15 eases (25.9％) showed T-cell marker. In this series there were 3 cases of large anaplastic subtype (Ki-1 lymphoma), which showed a strong positivity with BerH_2 (CD_(30)). Morphologically, there were some changes favourable to peripheral T-cell lymphoma, dust-like chromatin, medium size eosinophilic nucleoli, pale cytoplasm, vessel proliferation and eosinophilia, whereas the clumped chromatin, basophilic nuleoli and plasmacytoid differentiation were favourable to B large cell lymphoma.