漂白纸浆废水对鲫鱼(Carassius auratus)肝脏微粒体代谢酶的影响

Effects of Bleached-Kraft Pulp Mill Effluent on Microsome Enzyme in Carp (Carassius auratus) Liver

实验研究了一种漂白纸浆废水对鲫鱼(Carassiusauratus)的急性毒性以及肝脏微粒体尿二磷酸葡萄糖醛酸转移酶(UDPGT)和谷胱甘肽硫转移酶(GST)的影响,在生物化学水平探索低浓度漂白纸浆水对鱼类的危害.实验结果表明,该漂白纸浆废水的96hLC50和168hLC50分别为43.90±3.09%和29.66±6.20%.暴露30d后,10%和12%原废水处理组能显著地诱导鲫鱼肝脏微粒体酶GST的活性,然而,8%、10%和12%原废水处理组能显著地抑制UDPGT的活性(P<0.05).

In this study, the acute toxicity of BKME and the effects of BKME on UDPglucuronosyltransferase (UDPGT) and glutathionstranferase(GST) in carp (carassius auratus) liver were determined under laboratory conditions. The results of the acute toxicity test showed that 96 h and 168 h LC50 values associated with 95% CI were 43.90±3.09% and 29.66±6.20% respectively. It was found that there were significantly induced effects on hepatic GST when carp were exposed to different concentrations of Bleached Kraft pulp Mill effluent (10% and 12%) for 30 days. However, the exposure to BKME (8%,10% and 12%) resulted in a marked inhibition to UDPGT levels in carp liver.