猪资源家系中肉质性状基因定位和遗传效应分析

Detection of Quantitative Trait Loci for Meat Quality in Swine Resource Family

目的 研究和探讨猪肉质性状主基因的定位方法及定位区间 方法 以 3头英系大白公猪和 7头梅山母猪建立F2 资源家系 ,随机选留屠宰 1 4 7头F2 代个体 ,获得肉质性状表型数据。对家系内所有个体 1、 2、 3、4、 6和 7号染色体上的 4 8个微卫星位点进行PCR扩增、判定基因型。利用线性模型最小二乘法对肉质性状进行数量性状位点 (QTL)区间定位 ,利用置换法确定显著性阈值。结果 猪 4号染色体 (SSC4 )上肌内脂肪QTL接近染色体显著水平 ,达到建议水平。SSC2、 6和 7上定位了肌肉含水量QTL ,达到染色体显著水平 (P<0 0 5或P <0 0 1 )。肌肉含水量QTL均有印迹效应 ,梅山和大白猪各有增效基因。结论 QTL定位时 ,不同的资源群体 。

Objective To locate the genetic regions in the swine genome that are responsible for meat quality traits in pigs. Methods A resource population was developed by intercross method with three Large White and seven Meishan pigs.In subsequent generations,147 F 2 offsprings were recorded for meat quality traits,and genotyped for 48 microsatellite markers spanning six chromosomes.Association analyses were performed using interval mapping by regression under an outbred line-cross model.The F threshold values were determined by permutation. Results The QTL affecting intramuscular fat on chromosome 4 (SSC 4) reached the suggestive level and was near the threshold chromosome-wide level( P <0 05).On SSC2,6 and 7,significant QTL at chromosome-wide level (P <0 05 or P <0 01)for moisture were detected,which showed imprinting effects.Meishan and Large White pigs both increased moisture.Conclusions QTL results may be different because of different reference families.