一个上皮样肉瘤细胞株的分子细胞遗传学研究

Molecular Cytogenetic Studies of a Epitfaelioid Sarcoma Cell Line

对建成的一个上皮样肉瘤(ES)细胞株进行了染色体分析和DNA印迹实验研究。结果表明,该瘤株染色体众数45,丢失1个22号染色体;结构异常为del(3)(q21.1)以及5、6、9和12号衍生染色体。用定位于人染色体3q13.1-q22的pPCC41A2克隆作为DNA标识探针,在该瘤株DNA中检出PCCB基因的部分缺失。本研究结果提示del(3)(q21.1)及PCCB基因的改变可能与ES癌变有关。

Chromosome and Southern blot analysis of a epithelioid sarcoma (ES) cell line were performed. Chromosome analysis revealed that the chromosomal model number was 45, lost chromosome 22, and the structural abnormalities were del (3) (q21.l), del (7) (q32), del (22) (q13,1) and 4 derivative chromosomes from No. 5,6,9 and 12 in the cell line. Southern blot analysis detected the partial deletion of PCCB gene in the cell line DNA with the PPCC41A2 clone (3q13.1-q22) as a DNA marker. These results suggest that del (3) (q21.1) and PCCB gene change may be involved in the development of ES.