神经胶质瘤患者血清和脑脊液中β-G活性与β_2-MG含量的同步测定及相关性研究

THE SYNCHRONOUS DETECTION AND INVESTIGATION OF ACTIVITY OF β-G AND CONCENTRATION OF β_2-MG IN BLOOD SERUM AND CSF OF PATIENTS WITH NEUROGLIOMA

目的 :研究神经胶质瘤患者血清和脑脊液中 β -葡萄糖醛酸酶 (β -G)活性与 β2 微球蛋白 (β2 -MG)含量的相关性及临床意义。方法 :分别应用ELISA法放免法。结果 :神经胶质瘤患者血清和脑脊液中无论 β -G活性 ,β2 -MG含量均明显高于正常对照组 ,并随着恶性程度的递增而增加 ,且手术前后也有显著差异。结论 :血清和脑脊液中二种活性物质的变化呈一致性关系 ,血清和脑脊液中 2种活性物质的检测均有助于神经胶质瘤的诊断、治疗及预后判定 ,尤其脑脊液的检测是更为客观、敏感的指标。

Objective:To study the correlation and the clinical significance of activity of β-glucuronidase (β-G) and β 2-microglobulin(β 2-MG) in blood serum and cerebrospinal fiuid(CSF) of patients with glioma.Methods: Samples were analyzed by enzyme-linked immunosorbent assay(ELISA), radioimmunoassay respectively in this study. Result:The patients with glioma had significantly higher levels of β-G? β 2-MG in their serum and CSF than those of the control group, there was positive correlation to malignancy and significant difference between preopration and postoperation. Conclusion: There is concordance between the variation of the two active substance serum and CSF, the two active substance contents in serum and CSF is conducive to diagnosis, treatment and prognosis of glioma, especially the variety of them m CSF is more objective and sensitive.