肝硬化、肝癌患者血清转化生长因子β1和干扰素γ的测定及临床意义

CLINCAL EVALUATION OF SERUM TRANSFORMING GROWTH FACTOR β1 AND INTERFERON γ LEVELS IN THE PATIENTS WITH LIVER CIRRHOSIS AND HEPATIC CARCINOMA

目的 :探讨转化生长因子 β1(TGF - βl)和干扰素γ(IFN -γ)在肝硬化、肝癌中的表达及临床意义。方法 :采用酶联免疫吸附试验法 (ELISA)测定了 4 8例肝硬化、4 0例原发性肝癌、6例转移性肝癌及 2 0例正常对照者血清中TGF - β1和IFN -γ含量。结果 :原发性肝癌患者血清TGF - β1水平显著高于肝硬化组和正常对照组 ,血清IFN -γ水平明显低于肝硬化组和正常对照组 (P均 <0 .0 5 )。原发性肝癌组的血清TGF - β1水平显著高于转移性肝癌组 (P <0 .0 5 ) ,而两组IFN -γ水平无显著性差异 (P >0 .0 5 )。肝硬化及转移性肝癌患者血清TGF - β1和IFN -γ水平无显著性差异 (P均 >0 .0 5 )。肿块数目≥ 3个 ,直径≥ 3cm和AFP≤ 2 0ng/ml的原发性肝癌患者血清TGF - β1水平明显增高 ,血清IFN -γ水平显著降低 (P均 <0 .0 5 )。 2 0例原发性肝癌患者经介入或手术治疗后血清TGF - β1水平明显下降 ,IFN -γ水平明显上升 (P <0 .0 5 )。结论 :血清TGF - β1和IFN -γ可作为新的血清学标志应用于原发性肝癌的鉴别诊断和近期疗效的判断。

Objective:To study the significance of TGF-β1 and IFN-γ levels in patients with liver cirrhosis and hepatic carcinoma. Method:Serum TGF- β1 and IFN- γ concentrations were determined in 48 patients with liver cirrhosis and 46 patients with hepatic carcinoma and 20 cases of normal subjects by means of enzyme-linked immunosorbent assay system.Result:Serum TGF-β 1 levels of primary hepatic carcinoma were significantly higher than those in normal subjects, in patients with liver cirrhosis and metastatic liver cancer ( P <0.05); Serum IFN-γ levels of primary hepatic carcinoma were significantly lower than those in normal subjects and in patients with liver cirrhosis( P <0.05), no obviously correlation was found between patients with primary hepatic carcinoma and metastatic liver cancer. After treatment of primary hepatic carcinoma, the amount of serum TGF-β1 declined significantly, the amount of serum IFN-γ increased significantly. Conclusion: Serum TGF-β1 and IFN-γ might be candidates for two novel tumor marker for diagnosis and monitoring ofprimary hepatic carcinoma.